ABSTRACT
AIM and METHOD: The adhesion behavior of bone marrow stromal cells(BMSC) in children with acute lymphoblastic leukemia(ALL) to bone marrow mononuclear cells(BMMC) and Raji cells were investigated by MTT method. The expression of adhesion molecules ICAM-1 and VCAM-1 were detected by flow cytometry. RESULTS: The adhesion ability of BMSC in acute period of ALL to BMMC was lower than that of control group. The adhesion ability of BMSC in long term remission period of ALL to Raji cells higher than that of control group. The expression of ICAM-1 on the surface of BMSC in acute period of ALL is much lower than that of control group. CONCLUSIONS: The adhesion of BMSC to BMMC or tumor cells in children with ALL was abnormal. The abnormal adhesion behavior might be partially due to the changed expression of adhesion molecules on BMSC.
ABSTRACT
Objective To investigate cytotoxicity and its mechanism of bilirubin on different types of neurons of rat in primary culture. Methods Toxic effect of bilirubin on primary cultured neurons (at cerebellar oranule hippocampal and cortical neurons) was observed. Ncuron survival was detected by fluorescei diacetate (FDA) staining. Measurement of fluorescent intensity of intracellular dilirudin were performed by microspectroflurimetry imaging system. Results Biliruin induced cytotoxicity to different type neurons in a dose-dependent manner. Exposed at 8d to different concentrations of bilirubin (4.3 ?mol/L、8.6 ?mol/L and 17.1 ?mol/L); cerebellar gtanule neuron survival werc (50.1?3.1)%,(18.5?2.3)%, (2.0?2.4)%, cortical neuron survival were (90.7?7.8)%, (85.3?5.3)%,(74.5?2.4)%, and hippocampal neuron survival were (75?6)%, (62?5)%, (49?5)% respectively. The toxic effect of bilirubin on ccrebellar granule neuron was significantly higher than that on hippocampal and cortical neurons. The fluorescent intensity of bilirubin in gra- nule, hippocampal and cortical neurons were 33.4?2.3、10.7?3.0、21.9?4.3 respectively three hours after addition of bilirubin to the medium. Significantly higher bilirubin concentration was found in granule neurons than that in hippocampal and cortical neurons (P